目的 探讨胃癌组织中胸苷酸合成酶(TS)mRNA表达与培美曲塞或雷替曲塞药物敏感性之间的关系.方法 收集50例经病理确诊的新鲜人胃癌标本,采用三维微组织块培养法(HDRA)进行两种药物的体外敏感试验；实时荧光定量PCR检测对应胃癌石蜡组织中TS mRNA水平.结果 新鲜胃癌组织对培美曲塞或雷替曲塞的敏感性以及胃癌石蜡组织中TS mRNA水平均与临床病理特征无明显相关性.培美曲塞敏感组与耐药组的TS mRNA相对表达量分别为6.72±1.34和15.39±2.43(P =0.002)；雷替曲塞敏感组与耐药组的TSmRNA相对表达量分别为7.96±1.70和14.14±2.37(P=0.028).结论 胃癌石蜡组织TSmRNA水平与新鲜胃癌组织对培美曲塞或雷替曲塞的敏感性呈负相关,TS在胃癌应用新型抗叶酸代谢类药物个体化治疗中具有潜在的指导价值.%Objective To investigate the correlation between the thymidylate synthase(TS) mRNA expression levels and in vitro chemosensitivity of gastric tumor specimens to pemetrexed and raltitrexed. Methods Freshly-removed gastric tumor Specimens were collected from 50 patients. Chemosensitivities to pemetrexed and raltitrexed were evaluated by histoculture drug response assay (DHRA); quantitative RT-PCR (qRT-PCR) was adopted for TS mRNA detection. Results There was no significant association between clinical characteristics and in vitro chemosensitivity or TS mRNA levels. TS mRNA levels in pemetrexed-sensitive group and raltitrexed-sensitive group were significantly lower than resistant groups (pemetrexed: 6.72 ± 1.34us. 15.39 ± 2.43, P =0.002; raltitrexed: 7.96 ± 1.70 vs. 14. 14 ± 2.37, P= 0.028). Conclusion TS mRNA expression level in gastric cancer is negatively correlated with in vitro chemosensitivity to pemetrexed and raltitrexed in gastric cancer. TS has potential predicting value in individual therapy of new antifolic acid drugs.
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