Objective To investigate the prognostic value of DNA copy number alterations (CNAs) in predicting intrahepatic recurrence of HBV-associated hepatocellular carcinoma after hepateclomy. Methods The genomic CNA status in tumor tissue DNA was detected by high-resolution array comparative genomic hybridization (aCGH) in 47 HBV-associated HCC samples. The follow-up time of these patients was 3. 1-28.0 months. The CNAs were analyzed for their association with intrahepatic recurrence by Kaplan-Meier and Cox proportional hazards models. Results Univariate analysis of the association between CNAs and intrahepatic recurrence revealed that 16ql 1.2-22. 1 loss was significantly associated with recurrence (P =0.001). HCC cases with 16ql 1.2-22. 1 loss as compared to those without the loss had a4. 59-fold (95% CI = 1. 64-12. 84,P=0.004) increased hazard ratio ( HR) for recurrence. Mult-ivariate analyses including 16ql 1.2-22. 1 loss and tumor stage showed that 16ql 1. 2-22. 1 loss was an independent predictive factor for recurrence (HR = 4. 64,95% CI = 1. 65-13. 06, P = 0. 004). Conclusion These findings suggest that 16ql 1. 2-22. 1 loss is associated with intrahepatic recurrence of HBV-associated hepatocellular carcinoma after hepatectomy and may be used as a predictive marker.%目的 探讨利用DNA拷贝数变异(CNA)预测HBV相关肝细胞癌(HCC)术后肝内复发的可行性.方法 采用高分辨率微阵列比较基因组杂交技术(aCGH),对47例HBV相关HCC肿瘤细胞基因组DNA的CNA情况进行检测；通过Kaplan-Meier生存分析和Cox风险比例模型,评估CNAs对HCC术后复发的影响.结果 单因素生存分析结果显示,16q11.2-22.1是否丢失对HCC术后复发有显著影响(P=0.001),而基因组内其他CNAs均与复发无关.与16q11.2-22.1未丢失者相比,16q11.2-22.1丢失HCC患者的术后复发危险比(HR)为4.59(95％ CI=1.64 ～ 12.84,P =0.004).多因素Cox回归分析显示,16q11.2-22.1是否丢失是HCC术后复发的独立预测因素(HR =4.64,95％ CI=1.65～13.06,P =0.004).结论 染色体片段16q11.2-22.1是否丢失是预测HBV相关HCC术后肝内复发的有效指标.
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