This study was conducted to investigate the hydrolysis ability of different proteases from China market on degrading glycinin and β-conglycinin of SBM in vitro.This in vitro trial included 4 treatments with 3 replicates per treatment.SBM used as the nature substrate was the control treatment,SBM supplemented with 3 different proteases were designed as the other 3 treatments.The adding dosage of Protease DP100,Protease J and Protease K collected from different suppliers was 1％,0.25％,and 0.3％ respectively,which was 20 times recommendation dosage of each supplier.The content of glycinin and β-conglycinin of SBM was measured by commercial testing kit before and after enzymatic hydrolysis.The results of this trial demonstrated that the hydrolysis rate of glycinin in SBM supplemented with DP100,Protease J and Protease K was 73.3％,5％ and 5.9％ respectively,and the hydrolysis rate of β-conglycinin in SBM supplemented with DP100,Protease J and Protease K was 52.1％,8.4％ and 0％ respectively.In conclusion,different protease products have different effects on allergic protein of SBM.Compared with Protease J and Protease K,DP100 protease was more efficient in degrading glycinin and β-conglycinin of SBM in vitro.%本试验旨在体外评估几种蛋白酶对豆粕中球蛋白和β-伴球蛋白的降解效果,为饲用蛋白酶效果的快速评估提供依据.试验设4个处理(3个蛋白处理组和1个空白对照组),每个处理3个重复,蛋白酶DP100、蛋白酶J和蛋白酶K的添加量分别为1％、0.25％和0.3％,为各蛋白酶制剂产品推荐添加量的20倍.豆粕和豆粕加酶样品在相同的缓冲体系中酶解,用试剂盒检测豆粕酶解前后球蛋白和β-伴球蛋白的含量,进而评估不同蛋白酶对豆粕中球蛋白和β-伴球蛋白的降解效果.结果表明:豆粕中球蛋白和β-伴球蛋白的含量分别为13.47％和13.96％,占豆粕总蛋白含量的29.6％和30.3％;不同蛋白酶在体外对豆粕中球蛋白和β-伴球蛋白的降解幅度不同,蛋白酶DP100、蛋白酶J和蛋白酶K对豆粕中球蛋白降解率分别为73.3％、5％和5.9％,对豆粕中β-伴球蛋白的降解率分别52.1％、8.4％和0％.表明,蛋白酶DP100在体外对豆粕中球蛋白和β-伴球蛋白的降解效果明显优于蛋白酶J和蛋白酶K.
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