本研究主要探讨不同品种肉鸡PRKAA基因表达的规律及其与MyHC基因表达和肌内脂肪沉积的关系.选择体重一致的1日龄AA鸡、BJY鸡各90只,每品种随机分为6个处理,每处理15只,在相同营养标准和管理条件下饲养至1、56日龄进行屠宰,测定腿肌MyHC、PR KA4基因表达水平与肌内脂肪(IMF)的含量.结果表明:AA鸡和BJY鸡1日龄和56日龄的PRKAA1基因表达水平差异不显著(P＞0.05),而PRKAA2、MYHC基因表达水平及IMF沉积差异显著(P＜0.05).1日龄AA鸡MYHC_ⅡA基因、PRKAA2基因表达水平显著高于56日龄(P＜0.05),IMF含量都是1日龄显著高于56日龄含量(P＜0.05).相关性分析表明,PRKAA1基因表达与MYHC_ⅡB基因表达呈显著负相关,PRKAA2基因表达与MYHC_ⅡA基因表达呈显著正相关(P＜0.05),PRKAA2基因表达与IMF含量呈正相关.%To understand the effect of overfeeding on Mitogen-activated Protein Kinases 14(MAPK14)mRNA level, the goose MAPK14 gene partial sequence was cloned by RT-PCR in this study, and the goose MAPK14 gene expression in liver tissue was detected by Q-PCR. The result indicated that the obtained MAPK14 gene sequence was 951 bp coding 316 amino acid residues, the nucleotide sequence and amino acid sequence share 94.8%, 99.7% identity with the gallus MAPK14; The Q-PCR detection found that overfeeding caused the MAPK14 mRNA expression increased significantly in both two breeds, the mRNA level of MAPK14 in liver of Sichuan White goose was significantly higher than that of Landes goose between the control group and the overfeeding group. It was concluded that overfeeding induced the significant increase of MAPK14 mRNA level in goose liver tissues, and the effect of overfeeding was significant difference between the two breeds.
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