目的利用表面增强拉曼光谱(SERS)检测牛奶样品中的氨苄西林,对其进行定性及半定量分析.方法首先构建了新型氧化铝陶瓷SERS检测平台.其次对氨苄西林的拉曼特征峰进行了归属,并确定了最佳的实验条件.然后对水溶液中的氨苄西林进行了SERS检测,并获得了检测限.最后进行了牛奶样品中的氨苄西林的检测,并利用线性拟合实现了对加标牛奶样品中氨苄西林的半定量分析.结果 牛奶样品中的氨苄西林的检测限为10-3g/L,在10-3～10-1g/L浓度范围内,拉曼特征峰1000cmr-1的强度随牛奶样品中氨苄西林的浓度的线性关系为y-23408.93x+1801.47,相关系数为0.993,回收率在95.0％～110.0％之间.结论包括对牛奶样品的预处理过程(约5min)在内,整个SERS检测过程不超过7min.该方法对牛奶中的污染物进行定量和定性分析具有广阔的应用前景.%Objective To detect ampicillin in milk using surface enhanced Raman spectroscopy (SERS) and obtain qualitative and semi-quantitative analysis of ampicillin.Methods Firstly,a SERS detection platform of alumina ceramic substrate was constructed.Secondly,Raman characteristic peaks (1000cm-1) of ampicillin were assigned and the optimal experimental conditions were determined.Then the ampicillin in water was detected using SERS and the limit of detection was obtained.Finally,the ampicillin in milk was detected and the semiquantitative analysis of spiked milk samples of ampicillin was achieved using linear fitting.Results The limit of detection of ampicillin in milk samples is 1.0×103g/L.In the range of concentrations of 103～101g/L,the relationship of Raman peak intensity and concentration of ampicillin in milk can be expressed by a linear equation:y=23408.93x+1801.47(R2=0.993).The recovery rate was detected between 95.0％ and 110.0％.Conclusion The SERS detection process is less than seven minutes,including the pretreatment process of milk samples (about five minutes).This method has great potential for the quantitative and qualitative analysis of pollutants in milk.
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