Objective To investigate the probability of induction of clindamycin resistance in isolates of Staphylococcus from clinical laboratory and give the proofs of using antibiotic for clinical physicians. Methods K-B disk diffusion method was used to test erythromycin resistance and clindamycin resistance in isolates of Staphylococcus. D-test recommended by CLSI/NCCLS was used to examine the inducible resistance of erythromycin to clindamycin. Results A total of 68 strains (31 strains of Staphylococcus aureus, 37 trains of coagulate-negative Staphylococcus) were erythromycin-resistant but clindamycin-senstive in the 152 trains of Staphylococcus, 31 trains were positive in D-test (14 strains of Staphylococcus aureus, 17 trains of coagulate-negative Staphylococcus), the positive rates were 45.2% and 45.9%. None of the Staphylococcus was positive in D-test in other types of drug resistance. Conclusion The induction of clindamycin resistance is considerbly high. It suggested that the D-test should be routinly preformed in the erythromycin-resistant but clindamycin-senstive Staphylococcus in microbiological laboratories. The result of inducible clindamycin resistance should be reported so as to give the proofs for clinical physicians to used antibiotic correctly.%目的 了解我院临床分离的葡萄球菌红霉素对克林霉素诱导耐药的发生率，为临床医生正确选择药物提供依据。方法 采用K-B纸片方法检测葡萄球菌红霉素和克林霉素的耐药性。按照CLSI/NCCLS推荐的D-试验方法检测克林霉素诱导型耐药情况。结果 152株葡萄球菌中有68株(金黄色葡萄球菌31株，凝固酶阴性葡萄球菌37株)呈红霉素耐药、克林霉素敏感，其中31株D-试验阳性(金黄色葡萄球菌14株，凝固酶阴性葡萄球菌17株)，检出率分别为45.2％、45.9％，其他药敏表型未检出D-试验阳性菌株。结论 克林霉素诱导型耐药发生率高，临床微生物实验室对红霉素耐药、克林霉素敏感的葡萄球菌应常规进行D-试验，报道克林霉素诱导型结果，为临床医生正确用药提供依据。
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