To improve the microorganism oil extraction efficiency, microwave was adopted to break bacterium cells and the process of methanol extraction grease was researched. By measuring the cellular proteins release quantity, the better condition of microwave was 420 W in 2 min. Scanning electron microscopy analysis of the broken bacterium showed that there were holes and cracks in hyphae, and the structure was destroyed. So the effect of microwave broken was perfect. Then, by the reaction of methyl directly and orthogonal test, analyzing the influences of methyl rate from such factors as liquid-solid ratio, reaction temperature, reaction time, and KOH-methanol concentration. The result showed that the optimum conditions were as follows; the liquid-solid ratio (g/mL) 1:5, temperature 50 ℃ , KOH-methanol concentration 2. 5% , and time for 2 h. Dry bacteria oil rate was 51. 3%.%采用微波细胞破胞法处理深黄被孢霉,并对破胞后的菌体进行甲醇萃取油脂过程的研究.通过测量细胞内蛋白质释放量,确定微波处理的较佳条件为微波强度420W,反应2 min.对微波后菌体进行扫描电镜观察,菌丝出现孔洞和裂纹,结构完全被破坏,达到微波破胞的效果；再直接进行甲酯化反应,通过正交试验考察固液比、反应温度、KOH浓度以及反应时间对甲酯化得率的影响.结果表明微生物油脂甲酯化的最佳条件:固液比(g/mL) 1∶5,温度50℃,KOH -甲醇质量分数2.5％,时间2h,此条件下干菌含油率为51.3％.
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