The phenol or 4-biphenylol was used to modify the heme. The modified hemin group was recombined with apo-hemoglobin to prepare novel artificial hemoglobin. Absorption spectral sean demonstrated that the artificial recombined hemoglobins with modified heme were obtained. Experimental resultsshowed that the peroxidase-like activity of modified hemoglobins was enhanced compared with that of native hemoglobin. And the enzymatic activity of modified hemoglobin with 4-biphenylol modified heme was as about 1.6 folds than that of the native hemoglobin.%利用苯酚或对羟基联苯时血红蛋白的血红素辅基进行化学修饰,将修饰后的血红素与脱辅基血红蛋白进行重组得到新的血红蛋白.以光吸收扫描分析修饰血红素和重组血红蛋白,证明新的重组血红蛋白构建成功.酶活力测定表明,修饰血红素得到的重组血红蛋白的类过氧化物酶活性都比天然血红蛋白的酶活力高,用对羟基联笨修饰血红素得到的重组血红蛋白的酶活提高明显,约是天然血红蛋白的1.6倍.
展开▼
