目的：建立在唾液标本中检出EB病毒IgA抗体的方法，以快速、特异诊断鼻咽癌。方法：人工合成EBV-DNA酶及EA-D多肽片段，分别作为捕捉抗原，采用ELISA法检测鼻咽癌患者和健康人血清及唾液中IgA/EBV-DNA酶和EA-D抗体。结果：通过检出O.D值，经统计学处理，选出鼻咽癌诊断阈值为：血清O.D值≥0.3,唾液O.D值≥0.45（DNA酶与EA-D两者阈值一致）。鼻咽癌与健康者两组间DNA酶与EA-D的IgA滴度均为P<0.0001,有非常显著性差异，与病理确诊相比，阳性符合率为72.6％～77.3％。结论：证实EBV合成肽链作为捕捉抗原，唾液为标本，用ELISA法是可行的，方法简便，重复性好，价廉，但诊断符合率需进一步提高。%Objective：This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods：Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results：After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following：OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/EA-D titer in patients with NPC and the values in healthy volunteers,P<0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6％ － 77.3％ . Conclusion： The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.?
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机译：目的：建立在唾液标本中检出EB病毒IgA抗体的方法，以快速、特异诊断鼻咽癌。方法：人工合成EBV-DNA酶及EA-D多肽片段，分别作为捕捉抗原，采用ELISA法检测鼻咽癌患者和健康人血清及唾液中IgA/EBV-DNA酶和EA-D抗体。结果：通过检出O.D值，经统计学处理，选出鼻咽癌诊断阈值为：血清O.D值≥0.3,唾液O.D值≥0.45（DNA酶与EA-D两者阈值一致）。鼻咽癌与健康者两组间DNA酶与EA-D的IgA滴度均为P<0.0001,有非常显着性差异，与病理确诊相比，阳性符合率为72.6％～77.3％。结论：证实EBV合成肽链作为捕捉抗原，唾液为标本，用ELISA法是可行的，方法简便，重复性好，价廉，但诊断符合率需进一步提高。 %Objective：This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods：Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results：After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following：OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/ EA-D titer in patients with NPC and the values in healthy volunteers,P<0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6％ － 77.3％ . Conclusion ： The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.?
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