目的 观察肌萎灵冻干粉对大鼠脊髓损伤后的神经保护作用,并研究其对谷氨酸诱导的原代神经细胞凋亡的影响.方法 以质量30 g静态压迫硬膜外脊髓10 min建立大鼠脊髓损伤模型,于术后第1天、7天、14天进行各实验组神经功能评价,并观察损伤处脊髓形态学改变;建立低浓度谷氨酸诱导原代神经细胞的兴奋性毒性损伤模型,MTT法检测细胞活力,流式细胞仪检测细胞凋亡率,Western检测Bcl-2、Bax蛋白表达变化.结果 (1)肌萎灵冻干粉可明显提高脊髓损伤大鼠各时间段的BBB评分,改善后肢运动功能,减轻脊髓神经元水肿.(2)肌萎灵冻干粉可增强谷氨酸损伤后的神经细胞活力,缓解神经细胞损伤.(3)肌萎灵冻干粉能显著减少谷氨酸诱导的神经细胞凋亡.(4)肌萎灵冻干粉可不同程度的上调Bcl-2蛋白表达,下调Bax蛋白表达.结论 肌萎灵冻干粉可促进脊髓损伤大鼠后肢运动功能恢复,增强神经细胞活力,抑制谷氨酸诱导的神经细胞凋亡,调节凋亡相关蛋白表达,具有一定的神经保护作用.%Objective To observe the protective effects of Jiweiling lyophilized powder ( JWLF ) on the neuron of spinal cord injury ( SCI ) rats, and the influence on primary neuron apoptosis induced by glutamate in vitro, also explore the molecular mechanism of JWLF on the motor neuron disease ( MND ). Methods The SCI model of rats were developed with static compressive pressure of 30 g on the spinal epidural for 10 min, the neuron function was evaluated at 1 d,7 d and 14 d after the surgery and the morphologic changes of the damaged spinal were determined. The primary cortical neuron was damaged by glutamate at low concentration, then measure the viability and the expression of Bcl-2,Bax with methods of MTT, flow cytometry and Western blot. Results ( 1 )JWLF could obviously improve BBB rating score on SCI rats at different time and release the edema of spinal neurons. ( 2 )JWLF could enhance neuron activity in rat which suffered from glutamate and alleviate neuronal injury. ( 3 )JWLF remarkably inhibited the neuron apoptosis induced by glutamate. ( 4 )JWLF could balance the expression of Bcl-2 and Bax of neuron. Conclusion JWLF could promote hind limb motor function of SCI rats through enhancing the neuron activity and restraining the neuron apoptosis.
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