目的 探讨大黄素对大鼠肠缺血再灌注(I/R)损伤的保护作用.方法 36只雄性SD大鼠随机分成假手术组(A组)、缺血再灌注组(B组)、大黄素灌胃预处理组(C组)三组.A组仅开腹不夹闭血管,在术前2 h给予0.5%羧甲基纤维素钠溶液按1 ml/100g(大鼠体重)灌胃.B组术前处理同A组,术中用无创动脉夹夹闭大鼠肠系膜上动脉(SMA)制备肠缺血再灌注模型.C组在术前2 h按20 mg/kg的大黄素溶于等量0.5%羧甲基纤维素钠溶液,其余同B组.分别于灌胃前、缺血1 h时经股静脉取血,再灌注1 h后经下腔静脉采取血样.检测各组血清肠脂肪酸结合蛋白(IFABP)、肿瘤坏死因子-α(TNF-α)、内毒素的含量.结果 血清中IFABP的含量在小肠缺血1 h及再灌注1 h后,B组和C组的含量明显高于A组,但C组与A组相比没有显著差异.血清中TNF-α的水平在小肠缺血1 h时,B组和C组高于A组,而在再灌注1 h后,C组与B组相比明显下降.血清中内毒素水平在小肠缺血1 h及再灌注1 h后B组与A和C组相比升高,而C组与B组相比,C组明显低于B组.结论 肠缺血再灌注损伤可致血IFABP、TNF-α、内毒素升高,大黄素对小肠缺血再灌注损伤有保护作用.%Objective To investigate the protection of emodin on intestinal mucosa injury produced by intestinal ischemia -reperfusion ( I/R ) in rats. Methods Thirty six male SD rats were randomly divided into three groups: sham - operated controls ( Group A ), ischemia - reperfusion group ( Group B ) and emodin pretreated group ( Group C ). The superior mesenteric artery ( SMA ) was occluded for an hour then released for one hour to produce the intestinal I/R model in group B and group C. Group C was given emodin ( 20 mg/kg ) according to 1 ml/100 g ( rats'weight ) by oral administration before 2 hours of the operation. In the group A and B, similar volume of 0. 5% sodium carboxy methyl cellulose solution was administrated respectively. Blood samples were collected from femoral vein in each group before medicine administration and an hour after ischemia. After one hour of ischemia - reperfusion, blood samples were got from inferior vena cava. The serum levels of intestinal fatty acid binding protein ( IFABP ), tumor necrosis factor - a ( TNF - α ) and endotoxin were measured in each group. Results After an hour of ischemia and another hour of I/R, the levels of IFABP in group B and C were higher than that in group A, and there were no significant difference between group A and group C. The levels of TNF - α in group B and C were higher than that in group A at the time of intestinal ischemia, while after I/R, the concentration in group C decreased significantly compared with group B. After ischemia and I/R, the level of endotoxin in group B was obviously higher than that in group A and C, and the level of group C was significantly lower than that in group B. Conclusion The levels of IFABP , TNF - α and endotoxin in serum are increased after intestinal IR. Emodin can protect the intestinal structure and function against IR injury.
展开▼
