目的 研究低分子肝素干预大鼠酒精性肝病模型的作用机制.方法 56％ (V/v)的白酒平均以8 g/kg的剂量2次/d灌胃10周,初步制备大鼠酒精性肝病模型,成模后部分大鼠低分子肝素(100 IU/kg)皮下注射进行干预,4周后观察低分子肝素对大鼠的血清生物化学指标、肝组织中丙二醛及环氧化酶-2的影响.结果 与模型组比较,低分子肝素治疗组大鼠血清转氨酶、血脂及丙二醛含量明显下降(P＜0.05)；光学显微镜观察HE染色见脂肪变性、炎症反应程度明显减轻(P＜0.01),免疫组织化学染色及逆转录聚合酶链反应显示环氧化酶-2的表达均明显降低(P＜0.05).结论 低分子肝素通过改善脂肪代谢、抑制氧化应激及降低环氧化酶-2的表达而对酒精性肝病大鼠起到一定的保护治疗作用.%Objective To study the effect of low molecular weight heparin on alcohol-fed rats. Methods The model of alcoholic liver disease rats was made by alcohol gavage (infused with 56% of ethanol twice a day with a dose of 8 g/kg body weight for 10 weeks) , and then parts were treated with low molecular weight heparin (100 IU/kg). After 4 weeks, the effect of low molecular weight heparin on serum biochemical index, malondialdehyde and cyclooxygenase-2 in liver tissue was observed. Results Compared with model group, the levels of Iransaminases, lipids and malondialdehyde significantly decreased in low molecular weight heparin therapy group ( P <0. 05 ). Under the observation of optical microscope, the degree of liver steatosis, inflammation reaction were significantly reduced ( P < 0.01 ) . The levels of cyclooxygenase-2 in liver tissue were significantly lowered by immunohistochemical staining and semiquantitative reverse transcription-polymerase chain reaction. Conclusion Low molecular weight heparin has therapeutic effect on alcohol-induced liver disease rats by improving fat metabolism, reducing oxidative stress and decreasing the expression of cyclooxygenase-2.c
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