Objective:To investigate effects of a novel synthetic immunostimulator CH1b containing thiazolidin-4-one on the immunoregulation funotion of iNKT ( invariant nature killer T ) cells in active RA patients in vitro.Methods: Peripheral blood mononuclear cells( PBMCs) isolated from active RA patients were cultured with stimulation of α-Galcer and IL-2 in vitro and iNKT cells were then separated by using magnetic activated cell sorting( MACS) method with iNKT isolation kit.The cells were divided into three groups:control group (IL-2),α-Galcer group (IL-2+α-Galcer),CH1b group(IL-2 +CH1b).The effects of CH1b on the proliferation of iNKT cells in active RA patients were analyzed by using MTT assay.MILLIPLEX MAP Human Cytokine/Chemokine kit was used to evaluate the secretion of IFN-γand IL-4 in iNKT cells culture media.The expressions of IFN-γmRNA and IL-4 mRNA in iNKT cells were analyzed by RT-PCR.Results: Compared with control and α-Galcer group,the proliferation of iNKT cells of CH1b group were significantly higher( P<0.05).Compared with control,the ratio of IFN-γ/IL-4 in iNKT cells culture media in active RA patients of CH1b group were significantly lower (P<0.05).Compared with control,expressions of IFN-γmRNA and IL-4 mRNA were higher inα-Galcer group;compared with control,expressions of IL-4 mRNA were higher in CH1b group,while there were no obvious difference on expressions of IFN-γmRNA.Conclusion:CH1b was found to significantly stimulate the actived iNKT cells in active RA patients proliferation,promote the secretion of IL-4,and increase the ratio of IFN-γ/IL-4,promote the expression of IL-4 mRNA in iNKT cells in active patients.%目的：研究新型合成的含噻唑烷-4-酮的免疫调节剂（CH1b）对活动期RA患者iNKT细胞免疫调节功能的影响。方法：从RA患者外周血中分离得到单个核淋巴细胞（ PBMC），α-Galcer和IL-2体外刺激扩增后，经磁珠分选（ MACS）得到纯化的iNKT细胞，分成对照组（IL-2）、α-Galcer组（IL-2＋α-Galcer）、CH1b组（IL-2＋CH1b）。采用MTT法测定各组RA患者iNKT细胞增殖率；MILLIPLEX MAP Human Cytokine／Chemokine kit检测各组RA患者iNKT细胞培养上清中IFN-γ和IL-4的水平；采用RT-PCR方法检测各组RA患者iNKT细胞中IFN-γmRNA和IL-4 mRNA的表达水平。结果：与对照组和α-Galcer组相比，CH1b组iNKT细胞增殖率明显增加（P＜0.05）；与对照组相比，α-Galcer组IFN-γ和IL-4分泌水平明显增加（P＜0.05）， CH1b组IFN-γ和IL-4的分泌量也明显增加，IFN-γ／IL-4明显降低（P＜0.05）；与对照组相比，α-Galcer组IFN-γmRNA与IL-4 mRNA表达均明显增高，CH1b组IFN-γmRNA表达差异不明显，IL-4 mRNA的表达明显增高。结论：新型合成的免疫调节剂（ CH1b）可促进活动期RA患者活化的iNKT细胞增殖，并促进IL-4的分泌，上调IL-4 mRNA的表达，诱导iNKT细胞向Th2方向分化，对恢复RA病人体内免疫平衡具有重要意义。
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