AIM: To investigate the relationship between matrix metalloproteinase 2 (MMP -2) -735C→T polymorphism in the promoter region and coronary atherosclerosis (CAS) in Han population of China. METHODS: This study was conducted with a CAS group including 309 patients confirmed by angiography and 311 control healthy subjects. Genotype of -735C→T functional promoter polymorphism of the MMP-2 gene was determined by polymerase chain reaction - restriction fragment length polymorphism ( PCR - RFLP). The relationship between the polymorphism in MMP - 2 gene and CAS was analyzed. RESULTS: The frequency of CC genotype (86. 1 % ) in CAS group was significantly higher than that in control group (79. 7% ) , but the frequency of CT + TT genotype (13.9% ) in CAS group was significantly lower than that in control group (20. 3% ). The statistical difference between CAS group and controls was significant (x2 = 4. 398,P<0.05). The frequency of -735C in CAS group (92.6% ) was higher than that in control group (89. 1% ) and the frequency of -735T in CAS group (7. 4% ) was lower than that in control group (10. 9% ), with the statistical significant difference ( X2 = 4. 521, P < 0.05 ). The degree of stenosis in coronary artery did not significantly relate to the MMP -2 gene -735C→T polymorphism in the promoter region. CONCLUSION; The genetic polymorphism in MMP-2 promoter region ( -735C→T) is associated with the susceptibility to CAS in Han population of China. CC genotype and C al lele may be a genetic marker. The -735C→T polymorphism may be useful as a predictor of CAS.%目的:研究中国汉族人群基质金属蛋白酶2(MMP-2)基因启动子区-735C→T多态性与冠状动脉粥样硬化(CAS)的相关性.方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法,以309例经冠状动脉造影确诊为冠状动脉粥样硬化的患者为研究对象,检测MMP-2基因启动子区-735C→T多态性,以311例同期体检的正常人为对照组,比较两组间MMP-2基因多态性频率分布的差异,并结合冠脉造影情况,探讨MMP-2基因多态性与CAS发病及冠状动脉狭窄程度的关系.结果:CAS组CC基因型频率(86.1%)明显高于对照组(79.7%),而CT+TT基因型频率(13.9%)明显低于对照组(20.3%),两组之间的差异显著(P<0.05); CAS组C等位基因频率(92.6%)高于对照组(89.1%),T等位基因频率(7.4%)低于对照组(10.9%),两组差异显著(P<0.05).MMP-2基因-735C→T多态性与冠状动脉狭窄程度无关(P>0.05).结论:MMP-2基因-735C→T多态性可能与中国汉族人群CAS有关,MMP-2基因CC基因型和C等位基因可能是CAS遗传易感性的基因标记之一.
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