Aim To study the neurohumoral regulation effect of Zuojin Pill total alksloid ( ZJPTA ) on the gastric ulcer model induced by restraint water-immersion stress. Methods Restraint water-immersion stress method was adopted to establish gastric ulcer model. The ulcer index of rats and TNF-a production in gastric mucosal were determined. The levels of norepinephrine ( NE ), 5-hydroxytryptamine ( 5-HT ), dopamine ( DA ) and 5-Hydroxyindoleacetic acid( 5-HIAA ) in rat brain tissues including hippocampus, cortex, hypothal-amus, striatum and NE, epinephrine ( E ) in adrenal gland were detected by HPLC system with electrochemical detector. Results ZJPTA could shrink the size of ulcer area and reduce TNF-a production significantly. The pre-treatment with ZJPTA could decrease the concentrations of monoamine neurotransmitters compared with model group ( P < 0. 05 , P < 0. 01 ). The concentrations of NE and E in adrenal gland were increased in model group, and ZJPTA could inhibit the tendency ( P <0. 05 ,P <0. 01 ). Conclusion ZJPTA may play an important role in the anti-ulcer effect by regulating neurohumoral factors.%目的 左金丸是黄连和吴茱萸按6:1配伍组成,临床常用于胃溃疡的治疗.该文主要探讨左金丸总生物碱(ZJPTA)对束缚水浸应激性胃溃疡模型大鼠神经体液调节的影响.方法 采用束缚水浸法复制大鼠胃溃疡模型,测定ZJPTA对大鼠胃溃疡指数,胃黏膜TNF-α含量的影响.测定大鼠海马、皮质、下丘脑、纹状体等脑区中单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)及其代谢产物5-羟吲哚乙酸(5-HIAA)含量以及肾上腺组织中NE和肾上腺素(E)含量的影响.结果 预先口服ZJPTA可明显降低应激性胃溃疡大鼠的溃疡指数,明显降低胃黏膜中TNF-α的含量.各剂量ZJPTA可不同程度降低大鼠脑组织单胺类神经递质的浓度,与模型组比较差异具有显著性(P<0.05或P<0.01).模型组肾上腺组织NE和E含量明显升高,ZJPTA能明显抑制其升高(P<0.05或P<0.01).结论 ZJPTA可通过神经体液调节作用,对抗应激性胃溃疡损伤.
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