Objective To determine the effect of PPARγ agonist, Pioglitazone (PG) on the expression of ATP-binding cassette G1 (ABCG1) and liver X receptor α(LXRα) in RAW 264.7 macrophage-derived foam cells. Method 1) The in vitro cultured RAW264.7 macrophage was induced into foam cells by 50 mg/L oxidized low density lipopro-tein (ox-LDL) for 48 h. The morphology and the changes of the foam cells were stained by oil red O, and identified by light microscopy. 2) At 24 h after using different concentrations of Pioglitazone (0, 5, 10, 20, 30 μmol/L) on the foam cells, the content of cholesterol ester in the foam cells was measured by enzyme assay. The mRNA and protein ex-pression of ABCG1 and LXRαwas measured by RT-PCR and Western blot, respectively. Results PPARγagonist, PG could significantly reduce the content of cholesterol ester in the foam cells, and increase the mRNA and protein expression of ABCG1 and LXRα in RAW264.7 macrophage derived foam cells. Conclusion PPARγ agonist, PG may decrease the content of cholesterol ester in the foam cells by upregulating the mRNA and protein expression of ABCG1 and LXRα.%目的：观察PPARγ激动剂吡格列酮对RAW264．7巨噬细胞源性泡沫细胞胆固醇流出调节蛋白三磷酸腺苷结合核转运蛋白G1（ABCG1）、肝X受体α（LXRα）表达的影响。方法①体外培养RAW 264．7巨噬细胞，用50 mg/L的氧化型低密度脂蛋白胆固醇（ox-LDL）孵育48 h诱导成泡沫细胞，油红O染色并在光镜下鉴定泡沫细胞形态及变化。②以不同浓度吡格列酮（0、5、10、20、30μmol/L）作用泡沫细胞24 h后，酶法检测泡沫细胞内胆固醇酯的含量。用反转录-聚合酶链反应（RT-PCR）及免疫印迹法测定ABCG1、LXRα的mRNA及蛋白的表达。结果 PPARγ激动剂吡格列酮可显著减少泡沫细胞内胆固醇酯含量，并呈浓度依赖性增加RAW264．7巨噬细胞源性泡沫细胞ABCG1、LXRα的mRNA和蛋白的表达。结论 PPARγ激动剂吡格列酮减少泡沫细胞内胆固醇酯的含量可能是通过上ABCG1、LXRα的mRNA及蛋白表达来实现的。
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