Objective To study the expression of microvascular density (MVD) and vascular endothelial growth factor (VEGF) in hepatic implantation tumor after transcatheter arterial chemoembolization (TACE) combining recombinant human endostatin (rh-ES)and to evaluate the therapeutic effect and angiogenesis of the rat hepatic implantation tumor. Methods According to the method introduced by the documents,forty rats with implanted tumors were randomly divided into two groups,with twenty in each group. The treatment group were treated with TACE combining rh-ES. The control group were treated with TACE only. 7 days after embolism, the hepatic implantation tumors were taken out,and the growth rates of the tumors were calculated, then immunohistochem-istry were performed to demonstrate the expression of VEGF and MVD. Results The size of the tumor decreased after the treatment. There was no significant difference between treatment group and control group(PX). 05). The expressions of VEGF protein were detected by immunohistochemistry, whose expression value were(31 ± 17) % in the treatment group and (41 + 12)% in the control group,showing significant difference between the two groups (P<0. 05). The expression of MVD were (21 ±12)strips and (30+ 10)strips respectively in two groups,showing significant difference between them (P<0.05). Conclusion TACE combining rh-ES can significantly reduce the expression of VEGF and MVD and inhibit the angiogenesis of the tumor, which shows that rh-ES has inhibitory effects on antiangiogenesis after TACE.%目的 探讨重组人血管内皮抑素(rh-ES)联合肝动脉化疗栓塞术(TACE)对大鼠移植性Walker-256肝癌模型血管内皮生长因子(VEGF)表达和微血管密度(MVD)影响,评价rh-ES与TACE联合治疗对抑制大鼠移植性肝癌模型血管生成的疗效.方法 建立Wistar大鼠Walker-256肝癌模型,1周后随机分成治疗组和对照组,每组各20只.治疗组肝动脉内注入rh-ES-碘化油阿霉素乳剂,对照组肝动脉内注入碘化油阿霉素乳剂.术后1周取出肝内肿瘤组织,用免疫组织化学方法检测两组肿瘤组织内VEGF的表达和MVD.结果 两组术后肿瘤体积均缩小,两组比较差异无统计学意义(P>0.05).治疗组及对照组肿瘤组织VEGF表达分别为(31±17)%和(41±12)%,MVD分别为(21±12)条和(30±10)条,两组比较差异有统计学意义(P<0.05).结论 rh-ES与TACE联合治疗可以明显降低肿瘤VEGF表达和MVD,有一定抗移植性肝癌血管生成的作用.
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