Abstract: Fed-batch fermentation conditions of Brevndimonas diminut JNPP-NSS for L-proline produc- tion were studied. The results showed that the synthetic concentration of the L-proline was improved under the condi- tions as follows: the initial precursor glutamic acid concentration was 50 g/L, the optimal original glucose concentra- tion was 100 g/L, the glucose was fed with 3.0 g/( L · h) at 35 h, and the total glucose concentration was 120 g/L. As a result, the concentration of L-proline increased to 54.40 g/L with a yield of 0.45 g/g.%对缺陷短波单胞菌（Brevndimonasdiminut）JNPP—NSS产L-脯氨酸的补料分批发酵条件进行了研究。结果表明，发酵初始添加50g／L前体物质谷氨酸，以最适初始葡萄糖浓度100g／L，于35h以3．0g／（L·h）的流速补加葡萄糖，使总糖浓度达120g／L的补碳方式，L-脯氨酸的合成浓度有所提高。发酵结束，L-脯氨酸的终浓度提高到54．40g／L，底物葡萄糖对L-脯氨酸的转化率达到0．45g／g。
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