采用超声波提取法、微波辅助提取法、索氏提取法、回流提取法和浸渍提取法分别提取苦丁茶黄酮,以DPPH自由基清除能力、ABTS自由基清除能力、还原能力为抗氧化能力的评价指标,比较不同提取方法对黄酮含量和抗氧化活性的影响。结果表明,5种提取方法所得黄酮提取物都有强于维生素C的抗氧化活性,其强弱顺序是：索氏提取法>超声波提取法>微波辅助提取法>浸渍提取法>回流提取法。超声波法提取苦丁茶黄酮溶剂用量少、提取效率高,可作为制备抗氧化剂的首选方法。%Total flavonoids from Ilex kudingcha C. J. Tseng were extracted by different extraction methods (ultra-sound, microwave, Soxhlet, refluxing, maceration). Three assays for antioxidant capacity including DPPH scavenge activity, ABTS scavenge activity and reducing power were applied. The results showed that all the five extracts ex-hibited stronger antioxidant activity than vitamin C. The different extraction procedure affected the antioxidant activity of Ilex kudingcha C. J. Tseng. Soxhlet extraction showed the best antioxidant capacity, followed by ultrasound, mi-crowave, maceration and refluxing methods. To obtain less solvent consumption and higher extraction efficiency, ul-trasound extraction method should be chosen.
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