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首页> 外文期刊>Separation and Purification Technology >Optimizing the extraction of phenolic antioxidants from kudingcha made frrom Ilex kudingcha C.J. Tseng by using response surface methodology
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Optimizing the extraction of phenolic antioxidants from kudingcha made frrom Ilex kudingcha C.J. Tseng by using response surface methodology

机译：用响应面法优化从苦丁茶中提取的苦丁茶中酚类抗氧化剂的提取

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摘要

Kudingcha made from Ilex kudingcha C.J. Tseng is a potent antioxidant in vitro and rich in polyphenolic compounds. For better utilization of the resource, response surface methodology was used to optimize the extraction conditions of polyphenolic antioxidants from kudingcha. In the present study, the total polyphenol content (TPC) and IC_(50) of scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical were considered as the response values. Through optimization, the optimal extraction conditions with ethanol as extraction solvent were as follows: ethanol concentration 71%, extraction temperature 74 °C and time 61 min for TPC (235.24 mg chlorogenic acid/g); ethanol concentration 70%, extraction temperature 71 °C and time 60 min for potent scavenging activity on DPPH free radical (IC_(50) 480.87 μg/mL). While for water as a solvent, the optimal conditions were extraction temperature 91V and time 36 min for TPC (198.45 mg chlorogenic acid/g) and extraction temperature 92 °C and time 36 min for potent DPPH free radical scavenging activity (744.74μg/mL). Similar parameters were obtained for extraction with higher TPC and lower IC_(50) of scavenging activity on DPPH free radical when using either ethanol or water as solvent. Our results suggest that the same optimal conditions can be used to get extract of kudingcha with higher TPC and lower IC_(50) of scavenging activity on DPPH free radical with ethanol or water as solvent.
机译：由Ilex kudingcha C.J. Tseng制造的Kudingcha是一种有效的体外抗氧化剂，富含多酚类化合物。为了更好地利用资源，采用响应面法优化了苦丁茶中多酚类抗氧化剂的提取条件。在本研究中，总的多酚含量（TPC）和清除IC_（50）对2，2-二苯基-1-吡啶并肼基（DPPH）自由基的清除活性被视为响应值。通过优化，以乙醇为提取溶剂的最佳提取条件为：乙醇浓度为71％，提取温度为74℃，TPC反应时间为61分钟（绿原酸235.24 mg / g）。乙醇浓度为70％，提取温度为71°C，时间为60分钟，以有效清除DPPH自由基（IC_（50）480.87μg/ mL）。对于水作为溶剂，最佳条件是TPC的萃取温度为91V，时间为36分钟（绿原酸198.45 mg / g），DPPH自由基清除活性的最佳萃取温度为92°C，时间为36分钟（744.74μg/ mL）。 ）。当使用乙醇或水作为溶剂时，以较高的TPC和较低的IC_（50）清除DPPH自由基的活性获得了相似的参数。我们的结果表明，相同的最佳条件可用于以乙醇或水为溶剂，以较高的TPC和较低的IC_（50）清除DPPH自由基的苦丁茶提取物。

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