In order to screen the strains without producing biogenic amines,some microorganisms were isolated from the fermentation mash and rice soaking water of Chinese rice wine.It was found that three strains had no amino acid decarboxylase activity,and had a certain ability of biogenic amines degradation.Moreover,the metabolic characteristics of the three strains were analyzed.The results showed that OD600 of the three strains reached a considerable level in the culture medium of 2.50％Vol.In the 8.80％Vol culture medium,strains 5-4 and 8-3 were inhibited whose OD600 was significantly lower than that of strain 14-2-1.In 15.70％ Vol culture medium,the three strains were seriously inhibited and scarcely grew.The strain 14-2-1 identified as Lactobacillus plantarum had better acid production capacity,showing a certain superiority.Therefore,strain 14-2-1 could be used in Chinese rice wine brewing,and effectively reduce the biogenic amines of Chinese rice wine.%为筛选不产生物胺的乳酸菌菌株,对黄酒发酵醪液及米浆水中的微生物进行培养及与分离纯化,发现3株菌不具有氨基酸脱羧酶活性,但具有一定的生物胺降解能力.使用黄酒发酵液培养该3株菌,发现在2.50％ Vol的黄酒培养液中,3株菌能正常生长;在8.80％ Vol黄酒培养液中,菌株5-4和8-3生长能力明显弱于14-2-1;在15.70％Vol黄酒培养液中,3株菌均受到严重抑制.菌株14-2-1生长速率最快,繁殖最旺,经鉴定为植物乳杆菌,同时菌株产酸能力较强,产酸速率也较快,表现出一定的优越性,应用于黄酒酿造中,能有效降低黄酒中生物胺的含量.
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