In this paper,the type II diabetes model was established and the optimal dose of the pumpkin polysaccharide,pueraria fla-vonoids and pu-erh theabrowns were determined by measured blood glucose.On the basis,the fasting blood glucose (FBG),insulin (INS),insulin resistance (IR),high density lipoprotein (HDL)and low density lipoprotein (LDL)were also investigated.The FBG, INS,IR,HDL,LDL combined group was significantly better than the single component (P<0.05).The results revealed that the com-binations of pumpkin polysaccharide and pu-erh theabrowns, pueraria flavonoids and pu-erh theabrowns were effective.However, the pumpkin polysaccharide factorial analyses showed that the inter-actions of the three substances were interactive.The synergistic ex-periment showed that pumpkin polysaccharide combined with Pueraria lobata flavonoids or combination with tea-flavonoid signifi-cantly reduced TG content (P<0.05).The combination of theaflavin and pueraria flavonoids in reducing the content of FBG,TC,LDL was significantly better than flavonoids,and the effect of TG and INS was better than that of thepueraria flavonoids and pu-erh the-abrowns group (P<0.05).The molecular mechanism experiments showed that a single one and two of the three components played an important role in the expression of PI3K/Akt protein pathway.%通过建立Ⅱ型糖尿病小鼠模型,以血糖值的变化确定南瓜多糖、葛根黄酮、普洱茶褐素提取物降血糖的最佳剂量。在该基础上,通过对其空腹血糖值(FBG)、胰岛素(INS)、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)的测定,表明3种提取物单一组分及两两联合对Ⅱ型糖尿病小鼠糖脂代谢紊乱均有明显的改善作用,在FBG、INS、IR、HDL、LDL 指标上联合组显著优于单一组分(P<0.05)。析因分析结果表明,当3种物质两两联合时具有交互作用；协同试验发现,南瓜多糖与葛根黄酮联合或与茶褐素联合在降低 TG 含量上均与多糖组差异显著(P<0.05)；茶褐素与葛根黄酮联合在降低 FBG、TC、LDL 含量上效果显著优于黄酮组,在TG、INS方面效果显著优于茶褐素组和黄酮组(P<0.05)。分子机制试验表明,3种物质单一组分和两两联合在 PI3K/Akt 蛋白通路表达方面均有增强作用。
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