ABSTRACT:ObjectiveTo determine the frequencies and parameters of 18 short tandem repeat loci (STR) in Guangxi Yao and Miao population.MethodsDNATyperTM19 kit was used to detect the allelic frequencies of 18 STR loci (D5S818, D21S11, D2S1338, vWA, D8S1179, PentaE, D19S433, D18S51, FGA, D6S1043, D13S317, D12S391, D7S820, CSF1PO, D3S1358, D16S539, TPOX, TH01) in 70 unrelated Yao and 68 Miao individuals while polymerase chain reaction (PCR) ampliifcation and capillary electrophoresis were employed. The population genetic parameters were calculated.Results167 alleles were recognized with relevant frequencies ranging from 0.007 to 0.579 for Yao people and 0.007 to 0.537 for Miao people. No deviation was observed of the allele frequency from Hardy-Weinberg equilibrium (P>0.05). For statistical analysis of Yao and Miao people, their respective parameters were heterozygosity (H) 0.571 to 0.943 and 0.603 to 0.956, the discrimination power (DP) 0.767 to 0.971 and 0.782 to 0.968, the polymorphic information content(PIC) 0.520 to 0.890 and 0.580 to 0.870, the probability of paternity exclusion (PE) 0.258 to 0.884 and 0.294 to 0.910, total discrimination power (TDP) 0.99999999999999999997602 and 0.99999999999999999997338, and cumulative probability of paternity exclusion (CEP) 0.9999999788 and 0.9999999921.ConclusionsThe genetic polymorphism of 18 STR in Guangxi Yao and Miao population is essential data for human population genetics and forensic medicine.%采用 PCR扩增及毛细管电泳技术对广西瑶族、苗族人群138名个体进行18个短串联重复序列位点分析。在广西瑶族样本中检出167种等位基因，在广西苗族样本中检出159种等位基因，广西瑶族基因频率分布为0.007~0.579，广西苗族为0.007~0.537，所有基因座基因型频率均符合Hardy-Weinberg平衡( P＞0.05)。广西瑶族、苗族群体杂合度分别为0.571~0.943和0.603~0.956，个人识别能力为0.767~0.971和0.782~0.968，多态信息总量为0.520~0.890和0.580~0.870；非父排除率分别在0.258~0.884和0.294~0.910，累积个体识别力为0.99999999999999999997602和0.99999999999999999997338；累积非父排除概率为0.9999999788和0.9999999921。
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