利用线粒体DNA细胞色素b基因的421 bp部分序列对北屯、乌伦古湖、博斯腾湖3个野生河鲈（ Perca fluviatilis）群体和北湖、五家渠2个养殖河鲈群体序列多样性与种群遗传结构进行分析。结果表明：100个个体中检测到7个单倍型，变异位点10个，其中野生群体60个个体检测到9个变异位点和6个单倍型，养殖群体40个个体共检测到8个变异位点和4种单倍型；野生群体平均单倍型多样性和平均核苷酸多样性（ H d＝0.496±0.121， P i＝0.00253±0.00154）高于养殖群体（ H d＝0.416±0.127， P i＝0.00123±0.00113），分子方差分析揭示，98.74％的遗传变异性出现在种群内个体间。群体间的F ST 分析揭示野生群体和养殖群体分化程度较低（0.05＜FST＜0.15）。分子系统树和单倍型网络图分析也表明，河鲈单倍型间关系较近，野生群体和养殖群体不存在显著分化。%In this study , partial cytochrome b gene of the 421 bp mitochondrial sequences were used to analyze the genetic diversity and structure of the Preca fluviatilis from three natural populations (Beitun, Wulungu Lake and Bosten Lake ) and two cultivated populations ( North Lake and Wujiaqu ).The results showed that 10 variable nucleotide sites and 7 haplotypes were detected in 100 individuals.Among them, 9 variable sites and 6 haplotypes were found in 3 natural populations of 60 individuals.8 nucleotide sites and 4 haplotypes were found in 2 cultivated populations of 40 individuals.The average haplo-type diversity and nucleotide diversity of the cultivated populations ( Hd =0.416 ±0.127 , Pi =0.00123 ±0.00113 ) were just a little below that of natural populations ( Hd=0.496 ±0.121 , P i=0.00253 ±0.00154 ).AMOVA analysis demon-strated that 98.74%of the variance occurred among individuals within populations .The pairwise fixation index (F ST) re-vealed a low degree of genetic differentiation between the natural and cultivated populations ( 0.05展开▼
