Objective To evaluate effects of andrographolide on the expression of heat shock protein 70 ( HSP70 ) in HeLa cell line. Methods Effects of andrographolide on expression and mRNA of HSP70 in HeLa cells were assessed by Western blot and RT - PCR. Dual luciferase reporter assay was used to study effect of andrographolide on the transcriptional activity of HSP70. Results Andrographolide induced up-regulation of the mRNA and protein of HSP70; also enhanced the phosphorylation of HSP1, which enhanced the transcription of HSP70. Conclusion Andrographolide has the anti - stress effect, which is probably mediated with up - regulation of HSP70.%目的 观察穿心莲内酯对热休克蛋白70(HSP70)基因表达的影响,探讨其抗应激作用机制.方法 利用RT-PCR和Western blot方法分别检测穿心莲内酯对HeLa细胞中HSP70 mRNA和蛋白表达的影响;采用双荧光素酶报告基因技术,检测穿心莲内酯对HSP70启动子活性的影响.结果 RT-PCR和Western blot分析显示穿心莲内酯可诱导HSP70 mRNA和蛋白表达增多,同时引起热休克因子1的磷酸化修饰;报告基因技术显示穿心莲内酯可增强HSP70转录活性.结论 穿心莲内酯具有抗应激作用,其作用机制可能与增加HSP70表达有关.
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