Grifolafrondosa is a edible and medicinal fungi. We optimalizated the carbon and nitrogen sources for liquid culture of Grifola frondosa, then used the hot water extraction method, to extract the extracellular polysaccharides (EPS) from the culture media at 60 ℃ for 2 hours. The results showed that the suitable carbon and nitrogen sources were glucose and yeast. The best content of Grifola frondosa EPS was 9 g/L. This study laid a good foundation for further Grifola frondosa EPS study.%对灰树花(Grifola frondosa)菌丝体液体深层发酵条件进行了初步研究,在优化灰碳源和氮源等技术参数基础上,对发酵液中胞外多糖的形成规律进行了研究.研究结果表明,酵母浸粉和葡萄糖是灰树花生长较为适宜的碳氮源,发酵液中灰树花多糖含量最多可达9g/L.
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