采用融粘帚霉AS3.3987对葛根素进行微生物转化,生成的主要产物经分析鉴定是3′-羟基葛根素.采用高效液相色谱方法,以XDB-C18为色谱柱,甲醇:水(27∶73)为流动相,检测波长为254 nm,测得在160 r/min、30 ℃的转化条件下,底物加量为400 μg/mL,转化时间为36 h时,3′-羟基葛根素得率最高为93.2 mg/g葛根素.%The major product of microbial transfonnation of puerarin by Cliocladium deligue.scen.s AS3 .3987 was identified as 3' :hydroxypuerarin on the basis of its spectral data. 3' -hydroxypuerarin was directly determined by HPLC . Methanol and water (27: 73) was used as the mobile phase on C18 column and the UV detection wavelength was 254 nm . The maximum transformation rate obtained was 93 .2 mg 3' -hydroxypuerarin/g puerarin at 30℃, 160 r/min for 36 h with the substrate concentration of 400 μg/mL .
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