[目的]从海洋中筛选对农业病害真菌有较强拮抗作用的海洋微生物.[方法]以5种农业病害真菌为筛选模型,采用平皿拮抗法对分离获得的菌株进行初步筛选,对有活性的菌株再进行复筛,并选择活性高的菌株进行发酵工艺优化.[结果]初筛中有拮抗作用的海洋细菌有21株,进行复筛后菌株MB133在稻瘟菌平板上抑菌效果最明显,菌株MB025、MB026、MB133在油菜菌核病菌、辣椒炭疽病菌、番茄灰霉病菌平板上均存在抑菌效果.抑菌效果最明显的菌株MB133的优化发酵工艺:最适装液量为12%,最适接种量为2.0%,最适培养基pH 7.0.[结论]该研究为农用抗生素的推广和应用奠定了资源基础.%[Objective]The aim was to screen the marine microorganisms with strong antagonism to the agricultural disease fungi.[Method]Five kinds of agricultural disease fungi were selected as screening models,and the isolated strains were screened by Petri dish antagonism.The active strains were rescreened,and the strain with highest activity was selected to optimize the fermentation process.[Result]There were 21 strains of marine bacteria that had antagonistic effects in the initial screening.The bacteriostasis effect of MB133 strain on the plate ofP yricularia oryzae was most obvious after rescreening.The bacteriostasis effects of MB025,MB026 and MB133 strains on Sclerotinia sclerotiorum,Colletotrichum capsic and Botrytis cinerea were also found.The optimum fermentation process of MB133 strain was as followed:the optimum liquid amount was 12%,and the optimum inoculation amount was 2.0%,the optimum medium was pH 7.0.[Conclusion]This research has laid the resource base for the pop-ularization and application of agricultural antibiotics.
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