[目的]对类黄酮异戊烯转移酶基因在酿酒酵母中的表达进行研究.[方法]以粗毛淫羊藿为材料,对其类黄酮异戊烯转移酶基因(EaPT1)进行克隆,并构建重组质粒,将其转化至酿酒酵母INVSc1中表达,进行黄酮类底物粗酶检测.[结果]获得了大小约1200 bp的EaPT1基因；经SDS-PAGE检测,重组质粒转化后的酵母菌有疑似EaPT1的表达产物；粗酶检测中未能检测到EaPT1酶活性.[结论]为粗毛淫羊藿植物次生代谢,特别是类黄酮生物合成途径的解析奠定基础.%[Objective] The aim was to study the expression of flavonoid prenyltransferase-like genes in Saccharomyces cerevisia. [ Method] Using the Epimedium acuminatum as material, the flavonoid prenyltransferase-like gene (EaPTl) was cloned, and with the construction of re-combinant plasmid which expressed in Saccharomyces cerevisia, the crude enzyme was detected by flavonoid substrate. [ Result ] An about 1 200 bp EaPTl gene was obtained; through the SDS-PAGE detection, the Saccharomyces cerevisia which had be transformed in recombinant plasmid had as if EaPT1' s expression product; there was no EaPTX enzymatic activity in the crude enzyme detection. [ Conclusion ] It prepared the ground for the analysis of secondary metabolism of Epimedium acuminatum, specially the synthetic route of flavonoid organism.
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