[Objective] The paper was to understand whether abscisic acid (ABA) participates the defense effect of hosts against turnip crinkle virus (TCV). [Method] By external application of ABA and using the corresponding mutants,TCV coat protein contents of various plants were detected by western-blot,and the expression of defense-related genes was analyzed by RT-PCR. [Result] After external application of SA,JA,ET and ABA,only SA and ABA could significantly inhibit TCV accumulation. In arabidopsis-TCV interaction system,SA and ABA had antagonistic effect with each other,the defense effect against TCV induced by ABA was independent of SA pathway. [Conclusion] The plants could activate distinct signal pathways to adjust the defense of hosts against virus.%[目的]了解脱落酸(ABA)是否参与宿主对芜菁萎缩病毒(Turnip crinkle virus,TCV)的防御作用.[方法]外施ABA和选用相应突变体,利用Western-blot检测各植株中TCV外壳蛋白含量,并采用RT-PCR分析防御相关基因表达.[结果]外施SA、JA、ET和ABA后,仅SA和ABA对TCV积累有明显抑制作用.在拟南芥-TCV互作系统中,SA和ABA之间具拮抗效应,且ABA诱导对TCV的防御不依赖于SA途径.[结论]植物通过激活不同的信号途径调节宿主对病毒的防御.
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