[目的]建立一种应用C18键合磁珠固相萃取-液质联用检测蜂王浆中磺胺二甲嘧啶残留的方法.[方法]MCX固相萃取小柱联合C18磁珠对蜂王浆进行前处理,用HPLC -串联质谱仪对净化后样本进行检测.[结果]该方法的线性范围是5 ～ 80 μg/kg,相关系数0.993 1.添加水平为5、10、20 μg/kg时各种化合物的平均回收率范围为93％ ～ 104％,RSD值均不大于11.3％.[结论]该方法简单、快速,结合自动化设备,可实现蜂王浆样品中磺胺二甲嘧啶测定前处理的自动化.%[ Objective] To develop a method of C18-functionalized magnetic silica nanoparticles solid phase extraction- high performance liquid chromatography- tandem mass spectrometry for determination of sulfadimidine in royal jelly, [Method] The royal jelly samples were pretreated by MCX SPE column and C18-functionalized magnetic silica nanoparticles, the detection was carried out by HPLC-MS/MS. [Result] The detection method showed good linear relationship in the range of 5 - 80 μg/kg (r =0.993 1). The recovery ranges were between 93% - 104% with the relative standard deviations (RSD) below 11.3%. [Conclusion] The pretreatment method is simple, fast, time-saving, and easy to realize the automation of pretreatment with the automatic equipment.
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