[ Objective ] The aim was to study the extraction and determination of polyphenols in Camellia semiserrata seed cake. [ Method ] The orthogonal experimental design method L9 (34) was adopted to study the impacts of different solid-liquid ratio, extraction time, alcohol concentration and extraction temperature on extraction rate of polyphenols. [ Result ] The results showed thai the optimum extraction conditions were as follows: 40％ ethanol concentration, extraction time 2 h, extraction temperature 65 ℃, solid-liquid ratio 1: 25 and the content of polyphenol reached 3.5％ and the polyphenols of Camellia semiserrata seed cake significantly inhibited the growth of plant pathogenic anthracnose hypha.%[目的]研究南山茶籽饼粕中多酚类物质的提取及测定.[方法]采用L9(34)正交试验设计,研究了不同的料液比、提取时间、乙醇浓度和提取温度对多酚提取率的影响.[结果]最优浸提南山茶籽饼粕多酚工艺条件为:40%乙醇浓度、提取时间2 h、提取温度65 ℃、料液比1∶25.该条件下的多酚含量达到了3.5%,对植物病原真菌(炭疽菌)菌丝生长具有明显的抑制作用.[结论]该研究为南山茶籽饼粕的开发利用提供了理论依据.
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