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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Simultaneous determination and pharmacokinetic study of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS-MS
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Simultaneous determination and pharmacokinetic study of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS-MS

机译：LC-MS-MS同时测定比格犬提取物口服后比格犬血浆中茶多酚I，茶多酚II，茶多酚VI和茶多酚VII的同时测定和药代动力学

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摘要

For the first time, a rapid and specific LC-MS-MS method has been developed for the analysis of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII in beagle dog plasma. The method was applied to study the pharmacokinetics of Rhizoma Paridis extracts containing polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII. The analysis was carried out on an Agilent Zorbax XDB-C18 reversed-phase column (100×2.1mm, 1.8μm) by isocratic elution with acetonitrile and water (50:50, v/v). The flow rate was 0.25mL/min. All analytes including internal standards were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII ranging from 10 to 5000ng/mL. The intra-and inter-day precisions (RSDs) were less than 6.66 and 9.15%. The extraction recovery ranged from 95.53 to 104.21% with RSD less than 8.69%. Stability studies showed that polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII were stable in preparation and analytical process. The validated method was successfully used to determine the concentration-time profiles of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII.
机译：首次开发了一种快速，特异性的LC-MS-MS方法，用于分析比格犬血浆中的多叶素I，多叶素II，多叶素VI和多叶素VII。该方法用于研究含有多叶绿素I，多叶绿素II，多叶绿素VI和多叶绿素VII的Pariz提取物的药代动力学。该分析是在Agilent Zorbax XDB-C18反相色谱柱（100×2.1mm，1.8μm）上通过乙腈和水（50:50，v / v）等度洗脱进行的。流速为0.25mL / min。包括内标在内的所有分析物均通过电喷雾电离源的选定反应监控进行监控。对于多叶绿素I，多叶绿素II，多叶绿素VI和多叶绿素VII，线性响应范围为10至5000ng / mL。日内和日间精度（RSD）分别小于6.66和9.15％。提取回收率在95.53％至104.21％之间，RSD小于8.69％。稳定性研究表明，多叶素I，多叶素II，多叶素VI和多叶素VII在制备和分析过程中均稳定。经验证的方法已成功用于测定多叶素I，多叶素II，多叶素VI和多叶素VII的浓度-时间曲线。

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