[目的]用气相色谱-质谱联用法(GC-MS法)分析披针叶黄华中生物碱.[方法]采用醇类溶剂提取法,提出披针叶黄华总浸膏,经酸溶、碱化处理,再用氯仿萃取出总生物碱,利用GC-MS法对其化学成分进行分析鉴定.[结果]共鉴定出14个组分,其中有5种属于已有文献记载的生物碱,即d-鹰爪豆碱,金雀花碱,臭豆碱,羽扇豆碱,黄华碱.[结论]揭示了披针叶黄华的生物碱种类,为披针叶黄华的药用开发和毒性研究提供理论依据.%[Objective]The research aimed to analyze the alkaloid from Thermopsis lanceolate based on the gas chromatography-mass spectrometry (GC-MS). [Method]Alcohol solvent extraction method was used to extract the total extract of Thermopsis lanceolata, and it was treated by acid-soluble and alkalization. Then chloroform was used to extract the total alkaloids. Finally, GC-MS was used to analyze and identify the chemical composition. [Result]Fourteen components were identified, of which 5 composition were already documented, d-alkali Sparteine, Cytisine, stinky bean base, Lupanine , d-Thermopsin respectively. [Conclusion]The study showed the kinds of alkaloid from Thermopsis lanceolate, and provided the theoretical basis for its medicinal exploitation and toxicity study.
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