Objective:To compare the influences of fetal bovine serum ( FBS) DMEM medium ( containing 10% FBS, 20% FBS, and 40%FBS) on biological characteristics of isolated and purified human periodontal ligament stem cells in primary culture ( hPDLSCs) so as to provide theoretical and experimental basis for the research of hPDLSCs in vivo and in vitro. Methods:The primary culture and purification of hPDLSCs were performed with three groups of DMEM medium containing different concentrations of FBS, and cell morphology and growth characteristics were ob-served under inverted microscope. The cells were identified by immunocytochemistry of vimentin staining method, MTT colorimetric assay for cell proliferation. Result:HPDLSCs morphology was similar in the primary culture of DMEM medium with different concentrations of FBS in each group, with cell morphology and growth characteristics of normal display;the cell proliferation is stronger in the group containing 20 % FBS medi-um; three kinds of cultured hPDLSCs were phenotypically similar, all with positive expression of vimentin. Conclusion:DMEM medium containing different concentrations of FBS can amplify hPDLSCs in vitro, while maintaining their stem cell characteristics. The use of hPDLSCs containing 20% FBS culture conditions may be more suitable for its application in cell therapy and experimental research.%目的：：对比含10%、20%、40%胎牛血清( fatal bovine serun,FBS)的DMEM培养基对原代培养、分离纯化人牙周膜干细胞( human periodontal ligament stem cells,hPDLSCs)生物学特性的影响,为hPDLSCs的相关体内、体外研究提供理论和实验依据。方法：分别用3组含不同浓度FBS的DMEM培养基对hPDLSCs进行原代培养、分离纯化,在倒置显微镜下观察细胞形态、生长特征,用波形蛋白的免疫细胞化学染色方法进行细胞表型鉴定,MTT比色法检测细胞的增殖能力。结果：各组DMEM培养基原代培养分离的hPDLSCs形态相似,均显示正常的细胞形态和生长特征；其中含20%FBS培养基组细胞增殖力更强；3种培养条件培养的hPDLSCs表型相似,均阳性表达波形蛋白。结论：含不同浓度FBS的DMEM培养基均能在体外培养扩增hPDLSCs,并维持其干细胞特性。采用含20%FBS培养条件培养hPDLSCs可能更适合其在细胞治疗和实验研究中应用。
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