为分析FASLG的序列特征，结构特征及与受体FAS的结合位点，为后续研究作用机制提供理论基础和实验思路，从NCBI蛋白质数据库中获取FASLG的序列信息，并使用生物信息学软件ProtParam、 ProtScale、 SignalP 4.1、TMHMM Server v．2.0、ClustalX、SWISS-MODEL和AutoDock等进行序列分析。结果表明：FASLG序列全长为281个氨基酸，偏碱性，为不稳定的亲水性蛋白；为跨膜蛋白，不含信号肽；有一个FAS受体结合域。 FASLG的三维结构包含74个α螺旋，20个β折叠，56个延展片段，其余131个则全为无规则卷曲。 FASLG蛋白通过15个氨基酸与其受体FAS蛋白相互耦合，从而形成FAS／FASLG信号通路来诱导凋亡。可见得到的FASLG的序列特征、结构及受体结合位点，将对后续研究FAS／FASLG信号通路及miR-21和FASLG在结肠癌细胞中的作用提供新思路和方向。%To provide theoretical basis and experimental ways for follow-up study, we analyzed the sequence signature , structure and receptor binding sites of FAS protein .The sequence of FASLG protein was downloaded from NCBI database , and further studied by ProtParam, ProtScale, SignalP 4.1, TMHMM Server v.2.0, ClustalX, SWISS-MODEL and AutoDock.As results FASLG has 281 a-mine acids , which is alkaline , unstable hydrophilic and transmembrane protein .FASLG has no signal peptide and receptor binding cites of FAS.The 3D structure of FASLG consisted of 74 αhelices, 20 βsheets, 56 extending segments and 131 coils.The FASLG protein couples with its receptor protein of FAS by 15amino acids, then they will form the FAS/FASLG signaling pathways to induce cell apoptosis.In conclusion the sequence signature , 3D structure and receptor binding cites are obtained , which provides a new direc-tion for studying the FAS /FASLG signaling pathway and knowing the role of miR-21 and FASLG in colon cancer cells .
展开▼
