目的：研究中药滁菊中主要活性成分总黄酮对类风湿性关节炎(rheumatoid arthritis，AR)模型大鼠滑膜组织Wnt通路分泌型卷曲蛋白4(secreted frizzled-related protein 4，SFRP4)基因及SFRP4下游基因β-catenin和C-myc表达的调控作用。方法：采用大鼠关节炎评分法和足爪肿胀评分法评价滁菊总黄酮(Chuju total lfavonoids，CJTF)对AR模型大鼠的治疗作用；RT-PCR和Western印迹分别检测CJTF灌胃治疗对模型大鼠滑膜组织Wnt通路中SFRP4，β-catenin和C-myc基因mRNA和蛋白表达的调控作用。结果：经CJTF灌胃治疗后，模型大鼠关节炎评分、足爪肿胀评分均明显下降。模型大鼠滑膜组织中SFRP4基因mRNA和蛋白表达明显上调，而其下游基因β-catenin和C-myc基因mRNA和蛋白表达明显下调。结论：CJTF对AR模型大鼠有明显的治疗作用，其机制与以SFRP4为靶点，抑制AR模型大鼠滑膜组织Wnt通路的激活有关。%Objective:To determine the effect of Chuju total flavonoids (CJTF) on the secreted frizzled-related protein 4 (SFRP4) expression in Wnt pathway in rheumatoid arthritis (AR) model rats. Methods:hTe role of CJTF in the treatment of AR model rats was evaluated by rat arthritis score and paw edema score. The expression regulation of the SFRP4,β-catenin and C-myc in Wnt pathway in AR model rats was detected by RT-PCR and Western blot atfer CJTF gavage treatment. Results:Atfer CJTF treatment, the rat arthritis score and paw edema score in AR model rats were signiifcantly decreased when the AR model rats were treated with CJTF, the SFRP4 expression was signiifcantly up-regulated, while theβ-catenin and C-myc gene expression were signiifcantly down-regulated in AR model rat synovial tissues. Conclusion:CJTF has significant therapeutic effect and inhibitory effect on Wnt pathway activation by targeting SFRP4 in AR model rat synovium.
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