利用薄层色谱和GC-MS/FID结合的方法分析玉米胚芽毛油中的总甾醇、游离甾醇和甾醇酯的组成及含量,并且对甾醇酯上的甾醇和脂肪酸组成和含量进行了分析.结果表明:玉米胚芽毛油的总甾醇含量为(11.01±0.O1) g/kg,其中谷甾醇(59.04％)、菜油甾醇(21.16％)和谷甾烷醇(11.08％)是占比最多的3种甾醇.甾醇酯是玉米胚芽油甾醇的主要存在形式,占总甾醇的47.59％,游离甾醇占总甾醇的35.33％.谷甾醇(61.45％)、菜油甾醇(20.42％)、谷甾烷醇(11.26％)和豆甾醇(4.77％)是甾醇酯中的主要甾醇,亚油酸(57.81％)、油酸(21.09％)、棕榈酸(10.94％)和硬脂酸(2.86％)是甾醇酯中的主要脂肪酸.%The component and content of the total sterols,free sterols and sterol esters in corn germ crude oil were analyzed by the thin-layer chromatography and GC-MS/FID.The component and content of fatty acids and esters in sterol esters were also analyzed.The results showed that,the content of total sterols in corn germ crude oil was(11.01 ± 0.01) g/kg,and sitosterol (59.04％),campesterol (21.16％) and sitostanol (11.08％) were the predominant fractions of total sterols,most of the sterols in corn germ oil existed in the sterol form which accouns for 47.59％ while the free sterol accounts for 35.33％.Sitosterol (61.45％),campesterol (20.42％),sitostanol (11.26％) and stigmasterol (4.77％) were predominant sterols of sterol esters.The linoleic acid (57.81％),oleic acid (21.09 ％),palmitic acid (10.94％) and stearic acid (2.86％) were the main fatty acids of sterol esters.
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