The process parameters for extracting phytosterol from a raw material corn germ oil via mi-crowave assisted technique were optimized through the orthogonal experiments on the base of single factor test results. The results showed that the optimum extraction conditions were microwave temperature 50 ℃, microwave time 2.5 min, microwave power 600 W, of material-to-liquid ratio 1:13 g/mL, and the extrac-tion ratio 8.305 mg/g was obtained under these optimum conditions. The extracted crude sterol was used as an antioxidant to investigate scavenging hydroxyl free radicals (·OH) ability. The results showed that crude sterol from corn germ oil had stronger antioxidant activity.%以玉米胚芽油为原料，采用微波辅助提取植物甾醇，在单因素实验基础上，通过正交试验对提取工艺进行优化。结果表明，微波辅助提取玉米胚芽油中植物甾醇，优化条件为：微波温度50℃、微波时间2.5 min、微波功率600 W，料液比1：13 g/mL，此时植物甾醇提取率可达到8.305 mg/g。以正交优化工艺条件下提取的粗甾醇为原料，研究清除羟自由基（·OH）的能力来确定它的抗氧化活性。实验结果表明，玉米胚芽油中粗甾醇具有较强的抗氧化活性。
展开▼
