Objective To investigate the effects of fluvastatin on expression of Smad-3 in alkali-burned cornea in rats.Methods Alkali corneal burns were induced to right eye in 24 SD rats using 1 mol/L NaOH.Animals were then randomly received one of 3 treatments ( n =8 for each group): normal saline, fluvastatin, or dexamethasone.In 1~7 days, corneal neovascularization was examined with slit-lamp, and pathological changes were evaluated with HE staining . The expression of Smad-3 was detected with immunohistochemical staining .Results At 7 days after corneal burns , corne-al neovascularization was significantly less severe in rats treated with fluvastatin than in rats treated with normal saline ( P<0.05).Histopathological examines showed less neovascularization and inflammatory cells infiltration , and more orderly corneal structure .Immunohistochemical staining showed that the expression of Smad-3 was significantly lower in fluvastatin treated rats .Conclusion Fluvastatin can reduce the severity of corneal neovascularization , and the mechanism may be re-lated to the down-regulated expression of Smad-3.%目的：探讨氟伐他汀对大鼠角膜碱烧伤后Smad-3表达的影响。方法24只SD大鼠随机分为3组：生理盐水组、氟伐他汀组和地塞米松组，每组8只，用1mol/L的NaOH烧伤大鼠右眼，生理盐水组、氟伐他汀组和地塞米松组分别以生理盐水、氟伐他汀和1g/L地塞米松点眼。烧伤后1～7 d用裂隙灯观察角膜新生血管的生长情况，HE染色观察角膜组织病理学改变，免疫组化观察Smad-3的表达情况。结果烧伤后第7天，氟伐他汀组的角膜新生血管生长密度显著低于生理盐水组，差异有统计学意义， P ＜0．05。组织病理学检查结果提示，氟伐他汀组与生理盐水组相比，新生血管的形成明显减少，管腔小且稀疏，周围炎症细胞浸润明显减少，角膜组织结构明显整齐。免疫组化结果显示氟伐他汀组的Smad-3的表达情况显著降低。结论氟伐他汀能通过下调Smad-3的表达，来减轻大鼠角膜碱烧伤后新生血管的生成。
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