In order to extract the active substances from grape seeds at a low cost,the integrated extrac-tion of active substances,proanthocyanidins,grape seed oil and resveratrol,in grape seeds by using organic solvent were studied in this experiment.The results showed that the extracting rates of proanthocyanidins were not significantly different among the six extracting combinations,which were 163.196~164.740 mg/g.The highest extracting rate (231.047 mg/g)of grape seed oil was obtained from combination F, which was performed as grape seed oil (extracted with petroleum ether for two times at 55 ℃ and 30 min of each time,the ratio of material to liquid was 1∶10)→resveratrol (extracted with ethyl acetate for two times at 30 ℃ and 3 h of each time,the ratio of material to liquid was 1∶10)→proanthocyanidins (extracted with 70% alcohol for two times at 50 ℃ and 1 h of each time,the ratio of material to liquid was 1∶10).The highest extracting rate (3 9 .1 3μg/g)of resveratrol was obtained from combination E,which showed same while the difference was not significant compared with combination F.In addition,the pigment content was much higher in resveratrol concentrates for combination F,which led to the difficulty on subsequent decol-orization and purification.Considering the extracting rates of the three active substances and the subse-quent purification process,combination F showed the best performance at the extracting order of grape seed oil→resveratrol→proanthocyanidins.%为了以较低的成本充分提取葡萄籽中的活性物质,利用有机溶剂法对葡萄籽中的活性物质原花青素、葡萄籽油和白藜芦醇进行联合提取工艺研究.结果表明：有机溶剂法提取3种活性物质的6种不同组合中,原花青素的得率差异不显著,得率为163.196~164.740 mg/g；葡萄籽油得率最高的为 F组合[葡萄籽油(以石油醚为提取剂,料液比为1∶10,55℃浸提2次,每次30 min.)→白藜芦醇(以乙酸乙酯为提取剂,料液比为1∶10,30℃浸提2次,每次3 h)→原花青素(以70%乙醇为提取剂,料液比为1∶10,50℃下浸提2次,每次1 h)],得率达231.047 mg/g；白藜芦醇得率最大的为 E组合(3种成分的提取参数与 F组合相同,葡萄籽油→原花青素→白藜芦醇),得率为39.13μg/g,但与F组合的得率38.74 mg/g差异不显著,且E组合中获得白藜芦醇浓缩液色素含量较高,后续白藜芦醇的脱色纯化较为困难.综合3种物质的得率及后续的纯化工艺,F组合为最佳提取组合,即先提取葡萄籽油,其次提取白藜芦醇,最后提取原花青素.
展开▼
