To investigate the effects of the berberine in diabetic rats and the amyloid protein of serum βsample .Wistar male rats were fed with high-fat diet 8 weeks and injected strep-tozotocin (STZ) to establish T2DM rat model.According to blood glucose and weight , model rats were randomly divided into 5 groups:control group (Ctrl), model group (Model), high berberine dose group (H, 129.45 mg/kg· d), low berberine dose group (L, 64.72 mg/kg · d) and positive control pioglitazone group (PIO, 2.72 mg/kg· d).Continuous lavage 8 weeks after death , separated serum and brain tissue .Using the colorimetric method to ob-serve the rats fasting plasma glucose ( FPG) , insulin ( INS) parameters were determined by radioimmunoassay;enzyme-linked immunoassay measured Aβlevels in serum and brain tis-sue in rats.Compared with Ctrl , the rats with high -fat feeding 8 weeks have very signifi-cant weight gain (P<0.01;P<0.05); high-fat fed rats of FPG, INS 8 weeks, insulin sensitivity index (IAI) significantly lower (P<0.01;P<0.05) which induced insulin re-sistance in rats;mould diabetic rats after 8 weeks, the blood and the brain Aβat 125.61 ± 7.12 pg/mL and 999.22 ±85.08 pg/mL respectively, compared with the ctrl, it had signifi-cantly increase ( P<0 .01;P<0 .05 ); compared with model group , high berberine dose group reduced the content of brain and blood in Aβ(P<0.01;P<0.05) significantly.Rats were fed with high-fat diet 8 weeks and injected STZ (35 mg/kg) , it can successfully cop-ied diabetic rats model which were based on the IR of diabetic rats model with high glucose and high fat;high berberine doses can significantly reduce the content of brain and blood in Aβin diabetic rats .%通过建立高脂联合STZ诱导糖尿病大鼠模型，旨在探讨小檗碱对大鼠脑内与血中β样淀粉蛋白的影响．选用Wistar雄性大鼠，高脂饲料喂饲8周后，腹腔注射1％链脲佐菌素（35mg／kg）复制糖尿病大鼠模型．成模后，将模型大鼠按体重和血糖值随机分为5组：模型组、对照组、小檗碱高剂量组（H，129．45mg／kg· d）、低剂量组（L，64．72mg／kg· d）和阳性对照吡咯列酮组（PIO，2．72 mg／kg· d）．连续灌胃给药8周后处死，分离血清及脑组织．采用比色法观察大鼠空腹葡萄糖，放免法检测胰岛素含量；酶联免疫法检测大鼠血清和脑组织中Aβ水平．与空白组相比，大鼠高脂喂养8周体重呈非常显著性增加（P＜0．01）；高脂喂养8周时大鼠FPG、INS显著性升高（P＜0．01或P＜0．05），IAI显著性降低（P＜0．05），表明已诱发大鼠产生胰岛素抵抗．与空白组相比，糖尿病大鼠血中及脑内Aβ均显著性增加（P＜0．01或P＜0．05）；与模型组比较，药物干预8周后，小檗碱高剂量显著性降低脑内和血中Aβ的质量浓度（P＜0．01）．高脂喂饲8周联合STZ 35mg／kg能成功复制以IR为基础伴有高糖的大鼠模型；小檗碱高剂量能显著性降低糖尿病大鼠脑内与血中Aβ的质量浓度．
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