Objective To explore the expression of heme oxygenase - 1 ( HO - 1 ) in gastric mucosa from sham - operated and portal hypertensive rats and the possible role of carbon monoxide( CO ) in portal hypertensive gastropathy ( PHG ). Methods The Sprague Dawley rats were randomly divided into two groups,sham operation and model group ( PHG ). Fourteen days after a portal ligation or sham operation,the portal venous pressure was measured with physiology recorder, and the gastric mucosal blood flow volume was measured with the neutral red clearance method. Histopathological changes were evaluated by hematoxylin and eosin staining, the expression of HO - 1 in gastric mucosal specimens was immunohistochemically assessed, and expression of HO - 1 protein was assessed by Western blot analysis. Results Fourteen days after the surgery, morphology studies showed a number of mucosal capillaries and submucosal veins were dilated markedly without any inflammation. The portal pressure (16. 02 ±2. 78) mmHg in the PHG rats was significantly greater than that in the control rats ( 7. 83 ± 1. 05 ) mmHg( P 展开▼
