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首页> 中文期刊> 《吉林大学学报（医学版）》 >木葡聚糖基温敏水凝胶预防大鼠腹壁-肠壁再粘连及其对局部TGF-β1和CTGF表达的影响

木葡聚糖基温敏水凝胶预防大鼠腹壁-肠壁再粘连及其对局部TGF-β1和CTGF表达的影响

         
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摘要

Objective:To evaluate the anti-adhesion properties of xyloglucan(mXG)hydrogel in the L929 mouse fibroblasts,to establish the animal model of recurrent adhesion in the rats after adhesionlysis,and to investigate the prevention effect of mXG hydrogel in recurrent adhesion and its influence in the expressions of transforming growth factor-β1(TGF-β1)and connective tissue growth factor(CTGF).Methods:After seeding on the surface of mXG gel,the adhesion property of L929 mouse fibroblasts was detected with fluorescence staining.The rat models of recurrent adhesion were established after adhesionlysis.Fourty-eight SD rats were randomly divided into 3 groups respectively(n=16).In adhesion group,1 mL saline was injected into the abdominal wall and cecum of the rats;in commercial membrane group,the 2 cm×3 cm chitosan anti-adhesion membrane was used to cover the wound of abdominal wall of the rats;in mXG hydrogel group,1 mL 4% mXG hydrogel was injected into the abdominal wall and cecal wound of the rats,and abdominal surgery was ended after the complete formation of the hydrogel(3 min).Eight rats were killed in each group 7 and 14 d after the second operation,and the degrees of adhesion were evaluated and the histological changes were observed. Immunohistochemical staining was used to observe the expression levels of CTGF and TGF-β1.Results:A large number of L929 mouse fibroblasts proliferated well and exhibited a spherical morphology in control group;but in mXG hydrogel group,only very little L929 mouse fibroblasts were globular,showing the mXG hydrogel had good resistance to the adhesion of L929 mouse fibroblasts.Blunt separate adhesion surface formed in all of the rats after operation.7 d after the second operation,4-5 score adhesion with fibrous connective tissue hyperplasia formed in adhesion group;moderate adhesion formed in commercial membrane group with more connective tissue;most cecum and abdominal wall began to heal in mXG hydrogel group with less connective tissue.14 d after the second operation,more severe peritoneal adhesions presented in adhesion group with proliferated fiber connetive tissue and collegan;severe adhesions also presented in commercial membrane group;mild adhesion was found in two rats mXG group,the other rats had no adhesion,and the defects were almost completely recovered.On days 7 and 14 after the second operation,the mean adhesion score of the rats in mXG group was significantly lower than those in adhesion group and commercial membrane group(P<0.05).The expression levels of TGF-β1 and CTGF were increased with the increase of peritoneal adhesion scores and collagen deposition;the expression levels of TGF-β1 and CTGF were the highest in adgesion group and the lowest in mXG group.Conclusion:mXG hydrogels have good resistance to fibroblast adhesion,and mXG hydrogel is effective in reducing the formation of recurrent adhesion in the rats after adhesionlysis and can decrease the expression levels of adhesion-related factors TGF-β1 and CTGF.%目的:评价木葡聚糖基(mXG)温敏水凝胶对 L929鼠成纤维细胞的抗黏附性能,建立粘连松解术后再次粘连动物模型,探讨mXG温敏水凝胶对粘连松解术后再次粘连的预防作用及对转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)表达的影响.方法:将 L929鼠成纤维细胞接种于mXG温敏水凝胶表面,荧光显色检测其在凝胶表面的黏附性能.制备粘连松解术后再次粘连大鼠模型.48只 SD大鼠二次损伤完成后随机分成3组,分别为再粘连组,将1 mL生理盐水注射于大鼠腹壁和盲肠的创面;商业膜组,将2 cm×3 cm大小的壳聚糖防粘连膜覆盖于腹壁创面;mXG温敏水凝胶组,将1 mL 4% mXG温敏水凝胶注射至腹壁和盲肠的创面,待水凝胶完全形成(3 min)后关腹;每组16只.二次术后第7和14天,每组分别处死8只大鼠,对粘连程度进行评分及组织学观察,免疫组织化学法观察 TGF-β1和 CTGF表达水平.结果:对照组可见大量 L929鼠成纤维细胞贴壁生长,呈细长的梭形并有伪足的形成;mXG温敏水凝胶组在凝胶表面仅有非常少的 L929鼠成纤维细胞,呈圆球状.一次术后所有大鼠均形成了可用钝器分开的粘连区.二次术后第7天,再粘连组形成了4~5分粘连,纤维结缔组织大量增生;商业膜组大部分为中度粘连,纤维结缔组织增生较多;mXG组大部分大鼠未见粘连发生且盲肠及腹壁开始愈合,有少量纤维结缔组织增生.术后第14天,再粘连组大鼠腹腔粘连严重,纤维结缔组织及胶原大量增生;商业膜组形成了接近再粘连组的严重粘连;mXG组有2只大鼠发生轻微粘连,其余均未发生粘连,并且盲肠及腹壁创面恢复良好.术后第7和14天 mXG水凝胶组大鼠平均粘连评分明显低于再粘连组和商业膜组(P<0.05).免疫组织化学染色,TGF-β1和CTGF表达水平随大鼠腹膜粘连评分和胶原沉积升高而升高,在再粘连组表达最高,在 mXG温敏水凝胶组表达最低.结论:mXG温敏水凝胶具有优异的抗成纤维细胞黏附性能,可以有效预防粘连松解术后再次粘连的发生,并可减少粘连相关因子 TGF-β1和CTGF的表达水平.

著录项

  • 来源
    《吉林大学学报（医学版）》 |2018年第2期|223-228封2前插1|共8页
  • 作者

    孙红; 李硕峰; 崔菁; 张二帅; 李景武; 熊艳杰; 马丽桃; 姚芳莲; 车鹏程;

  • 作者单位

    华北理工大学基础医学院 河北省慢性病重点实验室 唐山市慢性病临床基础研究重点实验室;

    河北 唐山063210;

    华北理工大学基础医学院 河北省慢性病重点实验室 唐山市慢性病临床基础研究重点实验室;

    河北 唐山063210;

    华北理工大学基础医学院 河北省慢性病重点实验室 唐山市慢性病临床基础研究重点实验室;

    河北 唐山063210;

    天津大学化工学院高分子科学与工程系;

    天津300350;

    河北省唐山市人民医院腹盆外科;

    河北 唐山063001;

    华北理工大学附属医院病理科;

    河北 唐山063000;

    华北理工大学基础医学院 河北省慢性病重点实验室 唐山市慢性病临床基础研究重点实验室;

    河北 唐山063210;

    天津大学化工学院高分子科学与工程系;

    天津300350;

    华北理工大学基础医学院 河北省慢性病重点实验室 唐山市慢性病临床基础研究重点实验室;

    河北 唐山063210;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 腹部外科学;
  • 关键词

    木葡聚糖基温敏水凝胶; 再次粘连; 粘连松解术; 转化生长因子β1; 结缔组织生长因子;

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