Objective To compare the reverse effect of rapamycin and cyclosporine A on multidrug resistance of human breast cancer cell. Methods The drug resistance fold of MCF - 7/ADR was detected by MTT. Human breast cancer cells MCF - 7/S and MCF - 7/ ADR were separated to some groups respectively. In control group, ADM of different concentrations were added,and in experiment group, besides ADM of different concentrations, rapamycin or cyclosporine A of different concentrations were added,and in blank group,nothing was added. The inhibition rates were detected by MTT, then IC50 and reverse fold were calculated. Rapamycin or cyclosporine A were added to MCF -7/ADR with ADM, then concentrations of ADM in cells were detected by immunofluorescence. Results (1) The drug resistance fold of mcf-7/ADR was 12. 13. (2) For MCF -7/S, the reverse fold of rapamycin and cyclosporine A were 1. (3)For MCF - 7/ADR, cyclosporine A (10μmol/L) and rapamycin (lμmol/L) could decrease IC50 of ADM significantly. And as the concentration increasing, ICSO decreased, and reverse fold increased. (4) Cyclosporine A (10μmol/L) and rapamycin (lμmol/L) could increase the concentration of ADM in MCF - 7/ADR cells. With the increasing concentration of drugs, the concentration of ADM in cells increased. The concentration of ADM in cells with rapamycin was higher than that with cyclosporine A of the same concentration. Conclusion Rapamycin could reverse the drug resistance of MCF -7/ADR, and had better effect than cyclosporine A.%目的 比较雷帕霉素与环孢素A对人乳腺癌细胞的耐药逆转作用.方法 MTT法测定人乳腺癌耐药细胞株MCF-7/ADR的耐药倍数.乳腺癌敏感细胞株MCF - 7/S和耐药细胞株MCF -7/ADR各自分为:对照组:加入不同浓度阿霉素；实验组:加入不同浓度的阿霉素+不同浓度的雷帕霉素或者不同浓度的阿霉素+不同浓度的环孢素A;空白组.MTT法观察细胞抑制率,并算得半数抑制浓度,得出耐药逆转倍数.用免疫荧光法测得加入阿霉素的MCF - 7/ADR细胞株在不同浓度的雷帕霉素或者环孢素A作用下,细胞内的阿霉素浓度.结果 (1)MCF-7/ADR细胞株的耐药倍数为12.13.(2)对于MCF - 7/S细胞株,雷帕霉素和环孢素A的耐药逆转倍数均为1.(3)对于MCF - 7/ADR细胞株,10μmoL/L环孢素A使阿霉素的IC50显著降低(P＜0.05),逆转倍数为1.6,并且随着浓度的增加,IC50逐渐降低,逆转倍数有逐渐增高的趋势；1μmol/L雷帕霉素使阿霉素的IC50显著降低(P＜0.05),逆转倍数为1.6,并且随着浓度的增加,IC50逐渐降低,逆转倍数有逐渐增高的趋势.(4) 10μmoL/L的环孢素A和1 μmol/L的雷帕霉素,开始增加乳腺癌耐药细胞株MCF - 7/ADR细胞内的阿霉素浓度,并且随着浓度的增加,阿霉素浓度增加.相同浓度的药物作用下,雷帕霉素组的细胞内阿霉素浓度较环孢素A高.结论 雷帕霉素能逆转MCF - 7/ADR细胞株的耐药性,且效果较环孢素A好.
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