Objective:To observe the change of the level of AFP and Th1,Th2 cytokines in PeriPheral blood of Pa-tients with Primary hePatocellular carcinoma(PeC)by radiofrequency ablation(RFA). Methods:Chemiluminescence were used to detect AFP and ELISA were used to detect the level of serum IL - 2,IL - 4,IL - 6,IL - 10 IFN - γ and TNF - α in 46 cases with PeC using RFA before one day and after oPeration fourteen days and twenty - eight days. IL- 2,IFN - γ and TNF - α were used to rePresent cytokines of Th1 tyPe,and IL - 4,IL - 6 and IL - 10 to rePresent cytokines of Th2 tyPe. Results:Before RFA with Patients of PeC,AFP values in the serum was significantly higher than that in the healthy volunteer(P ﹤ 0. 05),the level of Th1 cytokines was significantly lower,the level of Th2 cyto-kines significantly higher than those of the healthy control grouP,resPectively(P ﹤ 0. 05). After RFA oPeration,AFP values in the serum was significantly lower,the level of Th1 cytokines were significantly higer,the level of Th2 cyto-kines was significantly lower than those of the healthy control grouP,resPectively(P ﹤ 0. 05). Conclusion:The level of Th1,Th2 cytokines were correlated with the change of AFP in Patients of PeC on RFA,might have an imPortant guid-ing significance for the Prognosis of PeC.%目的：观察原发性肝癌患者经射频消融（RFA）治疗后外周血甲胎蛋白（AFP）和 Th1、Th2细胞因子的变化，探讨射频消融术对原发性肝癌患者治疗效果与免疫功能间的关系。方法：应用射频消融治疗仪治疗原发性肝癌46例，采用化学发光方法检测 AFP 值，酶联免疫吸附（ELISA）方法检测患者在射频消融前和治疗后14天、28天外周血 Th1细胞因子[白介素（IL）-2、肿瘤坏死因子（TNF -α）、干扰素（IFN）-γ]及 Th2细胞因子（IL -4、IL -6、IL -10）的表达。结果：原发性肝癌患者射频消融治疗前外周血 AFP 值明显高于健康志愿者（P ﹤0.05），Th1细胞因子降低，Th2细胞因子升高，与正常对照组相比，差异有统计学意义（P ﹤0.05）。射频消融术后，大部分患者外周血 AFP 值明显降低，Th1细胞因子升高，Th2细胞因子降低，与正常对照组相比，差异有统计学意义（P ﹤0.05）。结论：Th1、Th2细胞因子的水平状态在原发性肝癌射频消融治疗后与 AFP 的变化存在相关性，可能对肝癌的预后有一定指导意义。
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