The antioxidant activities in vitro of different extracts from Incarvillea argute were detected by the deoxidization Fe3+ reaction system, salicylic acid method as well as DPPH free radical (DPPH o ) method in the present work. Extracts were obtained by treating material of I. argute with 80% aqueous ethanol, subsequently, were extracted with petroleum ether, chloroform, ethyl acetate and n-butanol. Different extracts were obtained respectively from corresponding extracts and water layer extract. Raw polysaccharide was obtained from water extract using ethanol precipitation. All of the extracts had strong deoxidization ability of Fe3+ , in which ethyl acetate extract showed the high deoxidization ability, but weaker than that of Vitamin C. The raw polysaccharide, n-butanol extract and water extract had weak scavenging effect on the hydroxyl free radical, while other extracts had no activity. The strong anti-DPPH free radical activity was observed from the different extracts, and ethyl acetate extract demonstrated the best scavenging effect on DPPH free radical. Therefore, the extracts from I. argute had antioxidant activities in vitro, especially anti-DPPH free radical effect, which provided scientific evidences for Miao Minzu medicine with anti-inflammatory effects.%采用80％乙醇和蒸馏水对毛子草进行提取,乙醇提取物加水分配后依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取相应的提取物和水层部分.水提部分经醇沉、干燥后得到毛子草粗多糖.再采用Fe3+还原能力、水杨酸法和二苯代苦味基肼自由基(DPPH·)法,比较毛子草不同提取物的抗氧化活性.结果表明:以上提取物均对Fe3具有较强的还原能力,其中乙酸乙酯萃取物的还原力最强,但弱于对照品Vc；除粗多糖、水提取物和正丁醇提取物有较弱的清除羟基自由基活性外,其余各提取物基本都没有清除羟基自由基活性；毛子草各提取物对DPPH自由基有较强的清除作用,以乙酸乙酯提取物清除能力为最强,但各提取物的清除能力均较Vc稍弱.说明毛子草各提取物都有一定的抗氧化活性,尤其是抗DPPH的活性最佳.
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