OBJECTIVE To investigate the volatile components of Alisma orientalis from three origins through three different extract methods by GC-MS. METHODS HS, SD and SFE-CO2 were accompanied with GC-MS to analyze the volatile components respectively. RESULTS HS-GC-MS affirmed 17 mutual components including δ-Elemene, β-Elemene and Beta-caryophyllene which are of high content. There were 14 mutual components identified by SD-GC-MS 14, of which Palmitic acid, Caryophyllene oxide and δ-Elemene are high contented. Besides, SFE-CO2-GC-MS got 7 mutual components, and n-Hexadecanoic acid, Alloaromadedrene and Ethyl linoleate are in a large quantity. CONCLUSION The volatile components of Alisma orientalis from different origins through different extract methods are different but connected. HS and SD are suitable for the further study into volatile components of Alisma orientalis.%目的 利用气相色谱-质谱联用技术(GC-MS)分析3种不同提取方法 获取的3种产地泽泻的挥发性成分.方法 顶空萃取(HS)、水蒸气蒸馏提取(SD)和CO2超临界流体萃取(SFE-CO2)3种方法 分别与气相色谱-质谱技术联用对泽泻挥发性成分进行分析.结果 HS-GC-MS法分析鉴别出17种共有挥发性成分,其中含量较高的有δ-Elemene(δ-榄香烯)、β-Elemene(β-榄香烯)、Beta-caryophyllene(β-石竹烯);SD-GC-MS法分析鉴别出14种共有挥发性成分,其中含量较高的有Palmitic acid(棕榈酸)、Caryophyllene oxide(氧化石竹烯)、δ-Elemene(δ-榄香烯);SFE-CO2-GC-MS法分析鉴别出7种共有挥发性成分,其中含量较高的有n-Hexadecanoic acid(棕榈酸)、Alloaromadedrene(别香树烯)、Ethyl linoleate (亚油酸乙酯).结论 不同提取方法 不同产地的泽泻药材挥发性成分既有联系又有区别.HS和SD法均较适宜用于泽泻挥发性成分的进一步研究.
展开▼
