Gold spheres of 12 nm prepared by reduction of sodium citrate are attached to retinol binding protein(anti-RBP).In pH=5.29 KH2PO4-Na2HPO4 buffer,the immune reaction between gold-labeled anti-RBP and retinol binding protein(RBP) is carried out,and the resonance light scattering intensity of 620 nm is increased significantly.ΔI620nm has a good linear relationship with concentration of RBP in 0.01-60 ng·mL-1,and the detection limits of RBP is 0.02 ng·mL-1.So it is a simple and sensitive method for the quantitative detecting of RBP in the clinical diagnosis with good results.%采用柠檬酸钠还原法制备了粒径约为12 nm的纳米金颗粒,并用其标记视黄醇结合蛋白抗体(anti-RBP).在pH=5.29的KH2PO4-Na2HPO4缓冲溶液中,纳米金标记anti-RBP可与视黄醇结合蛋白(RBP)发生特异性结合使纳米金颗粒凝集成网格结构,由于纳米金颗粒的表面等离子体共振效应,使其在620 nm处共振光散射信号显著增强.CRBP在0.1~60 ng·mL-1范围内与共振光散射增强值ΔI620 nm线性相关,检出限为0.02 ng·mL-1,用于定量分析人血清中的RBP,方法简单、灵敏,结果满意.
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