Rhodamine B served as an"on-off"fluorescent probe is used for the detection of hypochlorous acid selectively,and further applied for fluorescent imaging of exogenous hypochlorous acid in Hela cells successfully.The interaction mechanism of Rhodamine B and hypochlorous acid has been clarified by HPLC-MS and NMR.According to the previous literatures,there may be a change in the structure of Rhodamine B,which will build a spiro-Rhodamine B.However,in the presence of hypochlorous acid,hydrogen atom of xanthene of Rhodamine B is replaced with chlorine atom of hypochlorous acid,which produces corresponding chloride.The fluorescence quantum yields of chlorides are lower than that of Rhodamine B,and as the number of chlorine atoms increases,the fluorescence quantum yields of chlorides are lower more and more.%以罗丹明B为"开-关"型荧光探针分子对次氯酸进行高选择性检测,并将其成功用于海拉(Hela)细胞内对外源性次氯酸进行荧光成像,利用高效液相色谱-质谱联用和核磁共振等技术手段对罗丹明B和次氯酸的相互作用机理进行了研究.结果表明,罗丹明B与次氯酸作用后并不形成螺环化合物,而是次氯酸中的氯原子取代罗丹明B氧杂蒽片段上的氢生成氯代罗丹明B,其荧光量子产率相较于罗丹明B明显降低,并且氯代罗丹明B的荧光量子产率随着取代氯原子数的增多而下降.
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